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rab27a  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rab27a
    (a) RT-qPCR analysis of <t>RAB27A</t> . GAPDH was used as housekeeping gene. (b) Western blot analysis of RAB27A. Vinculin was used as loading control. (c) NTA analysis of particles in CM. (d) Representative images of morphology and SA-β-Gal staining (scale, 100 μm; scale in zoomed area, 200 μm). (e) Cell counting. (f) Cell size measurement. (g) Quantification of SA-β-Gal positive cells using X-gal substrate. (h) Quantification of SA-β-Gal positive cells using C 12 FDG substrate. (i) RT-qPCR analysis of CDKN1A . GAPDH was used as housekeeping gene. Significance was calculated using one-way ANOVA showing the exact p-value. Less than 0.05 was considered statistically significant (a, c-i, n=3; b, n=1).
    Rab27a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rab27a/Rab27A+Rabbit+mAb/bio_rxiv__64898__2026__03__25__713920-166-31-33
    Average 95 stars, based on 100 article reviews
    rab27a - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence"

    Article Title: Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence

    Journal: bioRxiv

    doi: 10.64898/2026.03.25.713920

    (a) RT-qPCR analysis of RAB27A . GAPDH was used as housekeeping gene. (b) Western blot analysis of RAB27A. Vinculin was used as loading control. (c) NTA analysis of particles in CM. (d) Representative images of morphology and SA-β-Gal staining (scale, 100 μm; scale in zoomed area, 200 μm). (e) Cell counting. (f) Cell size measurement. (g) Quantification of SA-β-Gal positive cells using X-gal substrate. (h) Quantification of SA-β-Gal positive cells using C 12 FDG substrate. (i) RT-qPCR analysis of CDKN1A . GAPDH was used as housekeeping gene. Significance was calculated using one-way ANOVA showing the exact p-value. Less than 0.05 was considered statistically significant (a, c-i, n=3; b, n=1).
    Figure Legend Snippet: (a) RT-qPCR analysis of RAB27A . GAPDH was used as housekeeping gene. (b) Western blot analysis of RAB27A. Vinculin was used as loading control. (c) NTA analysis of particles in CM. (d) Representative images of morphology and SA-β-Gal staining (scale, 100 μm; scale in zoomed area, 200 μm). (e) Cell counting. (f) Cell size measurement. (g) Quantification of SA-β-Gal positive cells using X-gal substrate. (h) Quantification of SA-β-Gal positive cells using C 12 FDG substrate. (i) RT-qPCR analysis of CDKN1A . GAPDH was used as housekeeping gene. Significance was calculated using one-way ANOVA showing the exact p-value. Less than 0.05 was considered statistically significant (a, c-i, n=3; b, n=1).

    Techniques Used: Quantitative RT-PCR, Western Blot, Control, Staining, Cell Counting

    (a) Representative images of morphology and SA-β-GAL staining (scale, 100 μm; scale in zoomed area, 200 μm). (b) Quantification of SA-β-GAL positive cells using X-gal substrate. (c) Flow cytometry plots of cells treated with conditioned media derived from control (CM-Scr), senescent (CM-Scr-S), or GW4869-treated senescent (CM-Scr-S+GW4869) cells, all of them expressing control Scrambled shRNA, and from RAB27A shRNA knockdown senescent cells (CM-shRAB27A #313-S). Significance was calculated using one-way ANOVA. Exact p-values are shown. Less than 0.05 was considered statistically significant (n=3).
    Figure Legend Snippet: (a) Representative images of morphology and SA-β-GAL staining (scale, 100 μm; scale in zoomed area, 200 μm). (b) Quantification of SA-β-GAL positive cells using X-gal substrate. (c) Flow cytometry plots of cells treated with conditioned media derived from control (CM-Scr), senescent (CM-Scr-S), or GW4869-treated senescent (CM-Scr-S+GW4869) cells, all of them expressing control Scrambled shRNA, and from RAB27A shRNA knockdown senescent cells (CM-shRAB27A #313-S). Significance was calculated using one-way ANOVA. Exact p-values are shown. Less than 0.05 was considered statistically significant (n=3).

    Techniques Used: Staining, Flow Cytometry, Derivative Assay, Control, Expressing, shRNA, Knockdown

    Related Articles

    Western Blot:

    Article Title: Secreted exosomes induce filopodia formation
    Article Snippet: Antibody , anti-ALK1/ACVRL1 (Rabbit polyclonal) , Abcepta , AP7807a , WB 1:1000. .. Antibody , anti-Rab27a (Rabbit monoclonal) , Cell Signaling , 69295 , WB 1:1000. .. Antibody , anti-Hrs (M-79) (Rabbit polyclonal) , Santa Cruz , sc-30221 , WB 1:1000.

    Article Title: Emerging role of oncogenic ß-catenin in exosome biogenesis as a driver of immune escape in hepatocellular carcinoma
    Article Snippet: Antibody , Anti-Cyclin D1 (mouse monoclonal) , Santa Cruz , sc-20044 , WB: 1/1000. .. Antibody , Anti-Rab27a (rabbit monoclonal) , Cell signaling , 69295 , WB: 1/1000, IF: 1/800, IHC: 1/100 pH6. .. Antibody , Anti-CD63 (rabbit polyclonal) , Sigma , SAB4301607 , WB: 1/500.

    Article Title: Exosomes are specialized vehicles to induce fibronectin assembly
    Article Snippet: .. Primary antibodies were: anti-CD63 (abcam ab68418, 1:500 for WB and ab8219, 1:100 for IF), anti-TSG101 (abcam ab30871, 1:1,000 for WB and 1:100 for IF), anti-flotillin (BD Biosciences 610820, 1:1,000 for WB), anti-HSP70 (sc-373867, Santa Cruz Biotechnology, 1:100 for IF), anti-GM130 (BD Biosciences 610822, 1:250 for WB), anti-Rab27a (Cell Signaling 69295, 1:1,000 for WB), anti-pan-Rab27 (OriGENE SKU AB7223, 1:500 for WB), anti-synaptotagmin-7 (Synaptic System 105173, 1:200 for WB), anti-fibronectin (Sigma-Aldrich F3648, 1:3,000 for WB and 1:300 for IF; BD Bioscience 610077, 1:5,000 for WB and 1:500 for IF; abcam ab23750, 1:500 for IF on tissues), anti-tenascin C (Millipore MAB1911, 1:500 for WB and IF; abcam ab108930, 1:1,000 for IF on tissues), anti-CD44 (abcam ab51037, 1:2,000 for IF on tissues), anti-integrin α4 (Cell Signaling 8440 (D2E1), 1:5,000 for WB), anti- integrin α5 (Santa Cruz Biotechnology 166665 (clone A11), 1:3,000 for WB), anti-integrin αv (abcam 208012, 1:1,000 for WB), anti-integrin β1 (BD Biosciences 610467 (clone 18), 1:1,000 for WB), anti-integrin β3 (Millipore MAB1974, 1:1,000 for WB), anti-integrin β5 (abcam 184312, 1:1,000 for WB), anti-syndecan-1 (BioRad MCA2459 (B-A38), 1:2,000 for WB, Cell Signaling 12922 (D4Y7H), 1:2,000 for WB, and Zimmerman lab 2E9 hybridoma supernatant, 1:4 for WB), anti-mouse syndecan-1 (BioLegend 142502 (281-2), 1:1,000 for WB), anti-RFP (Chromotek 6G6, 1:2,000 for WB) and anti-β-actin (Ac-74, Sigma, 1:5,000 for WB). .. HRP-conjugated goat anti-mouse IgG (W4021, 1:10,000 for WB) or goat anti-rabbit IgG (W4011, 1:10,000 for WB)were purchased from Promega.

    Immunohistochemistry:

    Article Title: Emerging role of oncogenic ß-catenin in exosome biogenesis as a driver of immune escape in hepatocellular carcinoma
    Article Snippet: Antibody , Anti-Cyclin D1 (mouse monoclonal) , Santa Cruz , sc-20044 , WB: 1/1000. .. Antibody , Anti-Rab27a (rabbit monoclonal) , Cell signaling , 69295 , WB: 1/1000, IF: 1/800, IHC: 1/100 pH6. .. Antibody , Anti-CD63 (rabbit polyclonal) , Sigma , SAB4301607 , WB: 1/500.



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    (a) RT-qPCR analysis of <t>RAB27A</t> . GAPDH was used as housekeeping gene. (b) Western blot analysis of RAB27A. Vinculin was used as loading control. (c) NTA analysis of particles in CM. (d) Representative images of morphology and SA-β-Gal staining (scale, 100 μm; scale in zoomed area, 200 μm). (e) Cell counting. (f) Cell size measurement. (g) Quantification of SA-β-Gal positive cells using X-gal substrate. (h) Quantification of SA-β-Gal positive cells using C 12 FDG substrate. (i) RT-qPCR analysis of CDKN1A . GAPDH was used as housekeeping gene. Significance was calculated using one-way ANOVA showing the exact p-value. Less than 0.05 was considered statistically significant (a, c-i, n=3; b, n=1).
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    (a) RT-qPCR analysis of <t>RAB27A</t> . GAPDH was used as housekeeping gene. (b) Western blot analysis of RAB27A. Vinculin was used as loading control. (c) NTA analysis of particles in CM. (d) Representative images of morphology and SA-β-Gal staining (scale, 100 μm; scale in zoomed area, 200 μm). (e) Cell counting. (f) Cell size measurement. (g) Quantification of SA-β-Gal positive cells using X-gal substrate. (h) Quantification of SA-β-Gal positive cells using C 12 FDG substrate. (i) RT-qPCR analysis of CDKN1A . GAPDH was used as housekeeping gene. Significance was calculated using one-way ANOVA showing the exact p-value. Less than 0.05 was considered statistically significant (a, c-i, n=3; b, n=1).
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    (a) RT-qPCR analysis of <t>RAB27A</t> . GAPDH was used as housekeeping gene. (b) Western blot analysis of RAB27A. Vinculin was used as loading control. (c) NTA analysis of particles in CM. (d) Representative images of morphology and SA-β-Gal staining (scale, 100 μm; scale in zoomed area, 200 μm). (e) Cell counting. (f) Cell size measurement. (g) Quantification of SA-β-Gal positive cells using X-gal substrate. (h) Quantification of SA-β-Gal positive cells using C 12 FDG substrate. (i) RT-qPCR analysis of CDKN1A . GAPDH was used as housekeeping gene. Significance was calculated using one-way ANOVA showing the exact p-value. Less than 0.05 was considered statistically significant (a, c-i, n=3; b, n=1).
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    (a) RT-qPCR analysis of <t>RAB27A</t> . GAPDH was used as housekeeping gene. (b) Western blot analysis of RAB27A. Vinculin was used as loading control. (c) NTA analysis of particles in CM. (d) Representative images of morphology and SA-β-Gal staining (scale, 100 μm; scale in zoomed area, 200 μm). (e) Cell counting. (f) Cell size measurement. (g) Quantification of SA-β-Gal positive cells using X-gal substrate. (h) Quantification of SA-β-Gal positive cells using C 12 FDG substrate. (i) RT-qPCR analysis of CDKN1A . GAPDH was used as housekeeping gene. Significance was calculated using one-way ANOVA showing the exact p-value. Less than 0.05 was considered statistically significant (a, c-i, n=3; b, n=1).
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    Image Search Results


    (a) RT-qPCR analysis of RAB27A . GAPDH was used as housekeeping gene. (b) Western blot analysis of RAB27A. Vinculin was used as loading control. (c) NTA analysis of particles in CM. (d) Representative images of morphology and SA-β-Gal staining (scale, 100 μm; scale in zoomed area, 200 μm). (e) Cell counting. (f) Cell size measurement. (g) Quantification of SA-β-Gal positive cells using X-gal substrate. (h) Quantification of SA-β-Gal positive cells using C 12 FDG substrate. (i) RT-qPCR analysis of CDKN1A . GAPDH was used as housekeeping gene. Significance was calculated using one-way ANOVA showing the exact p-value. Less than 0.05 was considered statistically significant (a, c-i, n=3; b, n=1).

    Journal: bioRxiv

    Article Title: Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence

    doi: 10.64898/2026.03.25.713920

    Figure Lengend Snippet: (a) RT-qPCR analysis of RAB27A . GAPDH was used as housekeeping gene. (b) Western blot analysis of RAB27A. Vinculin was used as loading control. (c) NTA analysis of particles in CM. (d) Representative images of morphology and SA-β-Gal staining (scale, 100 μm; scale in zoomed area, 200 μm). (e) Cell counting. (f) Cell size measurement. (g) Quantification of SA-β-Gal positive cells using X-gal substrate. (h) Quantification of SA-β-Gal positive cells using C 12 FDG substrate. (i) RT-qPCR analysis of CDKN1A . GAPDH was used as housekeeping gene. Significance was calculated using one-way ANOVA showing the exact p-value. Less than 0.05 was considered statistically significant (a, c-i, n=3; b, n=1).

    Article Snippet: Membranes were blocked with 5% milk solution and incubated with primary antibodies for GAPDH (sc-32233, Santa Cruz Biotechnology 1:1000), β-Actin (sc-8432, Santa Cruz Biotechnology, 1:1000), Vinculin (sc-76314, Santa Cruz Biotechnology 1:2000), RAB27A (#69295, Cell Signaling Technology, 1:1000), p53 (sc-6243, Santa Cruz Biotechnology, 1:1000), and p21 (#2947, Cell Signaling Technology, 1:1000), overnight at 4 °C.

    Techniques: Quantitative RT-PCR, Western Blot, Control, Staining, Cell Counting

    (a) Representative images of morphology and SA-β-GAL staining (scale, 100 μm; scale in zoomed area, 200 μm). (b) Quantification of SA-β-GAL positive cells using X-gal substrate. (c) Flow cytometry plots of cells treated with conditioned media derived from control (CM-Scr), senescent (CM-Scr-S), or GW4869-treated senescent (CM-Scr-S+GW4869) cells, all of them expressing control Scrambled shRNA, and from RAB27A shRNA knockdown senescent cells (CM-shRAB27A #313-S). Significance was calculated using one-way ANOVA. Exact p-values are shown. Less than 0.05 was considered statistically significant (n=3).

    Journal: bioRxiv

    Article Title: Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence

    doi: 10.64898/2026.03.25.713920

    Figure Lengend Snippet: (a) Representative images of morphology and SA-β-GAL staining (scale, 100 μm; scale in zoomed area, 200 μm). (b) Quantification of SA-β-GAL positive cells using X-gal substrate. (c) Flow cytometry plots of cells treated with conditioned media derived from control (CM-Scr), senescent (CM-Scr-S), or GW4869-treated senescent (CM-Scr-S+GW4869) cells, all of them expressing control Scrambled shRNA, and from RAB27A shRNA knockdown senescent cells (CM-shRAB27A #313-S). Significance was calculated using one-way ANOVA. Exact p-values are shown. Less than 0.05 was considered statistically significant (n=3).

    Article Snippet: Membranes were blocked with 5% milk solution and incubated with primary antibodies for GAPDH (sc-32233, Santa Cruz Biotechnology 1:1000), β-Actin (sc-8432, Santa Cruz Biotechnology, 1:1000), Vinculin (sc-76314, Santa Cruz Biotechnology 1:2000), RAB27A (#69295, Cell Signaling Technology, 1:1000), p53 (sc-6243, Santa Cruz Biotechnology, 1:1000), and p21 (#2947, Cell Signaling Technology, 1:1000), overnight at 4 °C.

    Techniques: Staining, Flow Cytometry, Derivative Assay, Control, Expressing, shRNA, Knockdown